Isothermal Amplification

"Loop-mediated isothermal amplification (LAMP) is a unique, temperature-independent technique for amplifying DNA that is simple to use, providing a visual display that is easy to read; additionally, the technique is robust and can be used at peripheral health centers."


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WHO Policy Guidance. The use of TB-LAMP for the diagnosis of pulmonary tuberculosis. August 2016

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Loop-mediated isothermal amplification (LAMP) amplifies previously determined genes and can be used to detect any pathogen. It enables the amplification at one temperature and the detection of target DNA faster and more specific than traditional PCR based methods. Similar to real-time PCR, generation of measuring signals occurs during or directly after a cycling process. The amplified products are detected visually by reading green fluorescence or by the measurement of turbidity in real-time.

The entire workflow consists only of four major steps:

  1. Sample transfer and lysis
  2. LoopampTM PURE DNA extraction or boil & spin method
  3. Loop-mediated isothermal amplification
  4. Result reading either visually or by using a turbidimeter

Its robustness, high sensitivity and the ease of use make LAMP an excellent and nearly failsafe solution to improve the diagnostic even in remote areas.

HUMAN provides LAMP testing systems for the screening of infections with bacteria belonging to the Mycobacterium Tuberculosis Complex (MTBC) as well as for the Malaria screening (Malaria Pan and Malaria Pan/Pf).

HUMAN provides the LAMP testing systems in cooperation with its partner Eiken Chemicals Co., Ltd.

Click here to learn more about EIKEN.