TB remains a global health issue
Tuberculosis remains one of the major causes of death worldwide. In 2017, there were an estimated 10 million TB cases according to the WHO, of which about 1.3 million led to death. The majority of these cases occur in low- and middle-income countries. Most frightening, however, is the fact that of the estimated 10 million cases, about 3.6 million remain undetected. Gaps between the estimated number of new cases and the number actually reported are due to underreporting of cases detected and underdiagnosis or misdiagnosis. The reasons for that are manifold.
Dr. Valerie Flore Donkeng Donfack
Institute Pasteur International
Eddie Samuneti Solo
Department of Pathology and Microbiology
University Teaching Hospital, Zambia
Current methods have their limitations
Microscopy provides only inaccurate results (low sensitivity) and requires two patient samples on two different days. Culture, the gold standard, offers better sensitivity and specificity, but the Patient often has to wait several weeks for its result, during which valuable time for treatment is lost. The best performance is achieved by molecular methods. In addition to a complex workflow and complicated evaluation of results, expensive equipment, air conditioning and a good infrastructure (continuous power with good quality, safety benches, etc.) are often required.
These requirements are often not to be found or met in smaller and peripheral laboratories. The WHO therefore recommends TB-LAMP as an alternative to smear microscopy.
Detect TB accurate and easily with TB-LAMP
The TB-LAMP workflow allows an easy DNA extraction and preparation of reaction mixes in a few steps with only a minimum of equipment and reagents. Reagent storage and shipment at room temperature as well as the excellent test performance make molecular pathogen testing available in rural areas with limited settings. Only lever to find the missing cases is the access to sensitive and reliable diagnosis. Therefore: TB-LAMP - It's time for a change!
“I do about five samples a day. With TB-LAMP I detect on average 2-3 samples as positive, which would be negative with microscopy."
Westlands Health Centre
“TB-LAMP is easier to interpret than smear microscopy. We also noted that the complexity of TB-LAMP was comparable to that of smear microscopy.”
Margaret Mutunga Nzina
Subcounty Medical Office of Health
“We love using the new TB-LAMP technology to do AFB testing: it is very quick and only takes about two hours for 14 samples per run – much faster than the other TB tests, which usually take at least a few days.
Another big advantage of TB-LAMP is that it gives very fast results with high sensitivity and specificity. A patient can know on the same day whether he has TB or not.
Lastly, the HumaLoop T is a simple small machine which requires little infrastructure."
Anderson Mugendi & Simon Chege
Kangemi Health Centre