TB remains a global health issue
Tuberculosis remains one of the major causes of death worldwide. In 2017, there were an estimated 10 million TB cases according to the WHO, of which about 1.3 million led to death. The majority of these cases occur in low- and middle-income countries. Most frightening, however, is the fact that of the estimated 10 million cases, about 3.6 million remain undetected. Gaps between the estimated number of new cases and the number actually reported are due to underreporting of cases detected and underdiagnosis or misdiagnosis. The reasons for that are manifold.
Dr. Valerie Flore Donkeng Donfack
Institute Pasteur International
Eddie Samuneti Solo
Department of Pathology and Microbiology
University Teaching Hospital, Zambia
Current methods have their limitations
Microscopy provides only inaccurate results (low sensitivity) and requires two patient samples on two different days. Culture, the gold standard, offers better sensitivity and specificity, but the Patient often has to wait several weeks for its result, during which valuable time for treatment is lost. The best performance is achieved by molecular methods. In addition to a complex workflow and complicated evaluation of results, expensive equipment, air conditioning and a good infrastructure (continuous power with good quality, safety benches, etc.) are often required.
These requirements are often not to be found or met in smaller and peripheral laboratories. The WHO therefore recommends TB-LAMP as an alternative to smear microscopy.
Detect TB accurate and easily with TB-LAMP
The TB-LAMP workflow allows an easy DNA extraction and preparation of reaction mixes in a few steps with only a minimum of equipment and reagents. Reagent storage and shipment at room temperature as well as the excellent test performance make molecular pathogen testing available in rural areas with limited settings. Only lever to find the missing cases is the access to sensitive and reliable diagnosis. Therefore: TB-LAMP - It's time for a change!
“TB-LAMP is a DNA-based method for quick TB diagnosis. It is fast, specific and has replaced microscopy because of its built-in heating block and UV light for reading sample results. It can be used in the remotest places because it's portable.
EpiConsult Clinic & Diagnostic Centre Ltd.,
“When I attended the training, Iunderstood that TB-LAMP is beneficialfor patients and technicians. Previously,the patient's first and second sputumwas used in microscopy, but now, withthe sensitivity of TB-LAMP, you onlyneed to take the sputum once. Withmicroscopy, it was difficult to handle 50samples per day. This technique allowsus to test 14 patients in one run and upto 70 in one day. For me, TB-LAMP isthe best method for rapid detection oftuberculosis.”
Joel Wepngong Tabah,
Baptist Hospital Mutengene,
“I like the HumaLoop T and the easyhandling very much. As a journey fromthe capital Luanda to my lab takes about3 days by road, it is especially importantfor us to have a reliable device thatrequires only a minimum service every12 months.”
“The high sensitivity, high throughputand the reliability of the assay,even under the challenging climateconditions, have convinced us. Withthis novel technology, we have theopportunity to find more tuberculosiscases than with traditional methods.”
Dr. Lal PathLabs,
“We love using the new TB-LAMPtechnology: it is very quick and onlytakes about two hours for 14 samplesper run. Thus a patient gets clarity onthe same day whether he has TB or not.TB-LAMP provides very reliable resultswith a high sensitivity and specificity.Lastly, the HumaLoop T is a simplesmall machine which requires littleinfrastructure.”
Anderson Mugendi & Simon Chege,
Kangemi Health Center,