From Microscopy to LAMP: Innovative Molecular Method is Paving the Way for Accurate, Robust and Easy-to-Use Detection of TB
Be a part of the WHO's global strategy to eliminate TB. Replace smear microscopy with the novel LoopampTM molecular diagnostic system recently recommended by WHO. With its fast, robust and easy availability LoopampTM offers an attractive diagnostic prospect in the elimination of TB.
Still Unmet Diagnostic Needs
Nearly a century since Robert Koch's breakthrough discovery, tuberculosis (TB) continues to kill over 1.5 million people every year. TB is one of the world's biggest threats, ranking alongside with HIV, and a leading cause of death worldwide. Part of the problem is a persisting major gap in TB diagnostics. Matters are further complicated with an alarming rise in the number of multi-drug resistant TB cases and HIV + TB coinfection cases. Early and effective detection of M. tuberculosis, particularly in smear-negative TB, is needed for global TB control and elimination.
To date the most common method for detecting TB is the sputum smear microscopy. While microscopy requires minimal biosafety standards it has certain drawbacks that present a major hurdle to the early and accurate diagnosis of TB:
- Low sensitivity, especially but not only in children and patients with HIV coinfection
- Lack of trained personnel
- Difficult to distinguish between Mycobacterium tuberculosis complex and other non-tuberculosis mycobacterium
The Solution: Embrace New Molecular Diagnostic Technologies
Diagnostic tools using molecular technologies such as nucleic acid amplification tests (NAATs) are the most advanced. Overcoming major restrictions of NAAT loop mediated isothermal amplification (LAMP) used in the novel LoopampTM system is a rapid, robust and easy-to-perform NAAT with a unique capability to be applied at remote and/or point of care settings. LoopampTM is especially suited for peripheral-level laboratories.
Features that make LoopampTM the diagnostic platform of choice especially in remote settings:
- Short turnaround (<1 hour)
- Flexible throughput (16-96/tests/run)
- High sensitivity and specificity
- Basically maintenance-free consolidated platform
- Robust to inhibitors and reaction conditions that usually adversely affect PCR methods
The "End TB strategy" targets are set for 2035. The goal is to reduce the absolute number of TB deaths by 95% and to reduce the TB incidence rate by 90%, compared to the baseline set for 2015. Diagnostic tools play a critical role to achieve these targets. Update your laboratory with LoopampTM and be a part of the global strategy to combat TB.
- World Health Organization., Global tuberculosis report 2015. 20th ed ed. 2015, Geneva: World Health Organization. 192 p.
- Nnadi, C.D., et al., Mind the gap: TB trends in the USA and the UK, 2000-2011. Thorax, 2016. 71(4): p. 356-63.
- Ou, X., et al., Diagnostic Accuracy of the PURE-LAMP Test for Pulmonary Tuberculosis at the County-Level Laboratory in China. PLoS ONE, 2014. 9(5): p. e94544.
- Mitarai, S., et al., Evaluation of a simple loop-mediated isothermal amplification test kit for the diagnosis of tuberculosis. Int J Tuberc Lung Dis, 2011. 15(9): p. 1211-7, i.
- WHO Press Release: WHO recommends new tuberculosis test - Robust, rapid DNA-based test can serve as an alternative to sputum smear microscopy